A two-dimensional mutate-and-map strategy for non-coding RNA structure
نویسندگان
چکیده
منابع مشابه
A two-dimensional mutate-and-map strategy for non-coding RNA structure.
Non-coding RNAs fold into precise base-pairing patterns to carry out critical roles in genetic regulation and protein synthesis, but determining RNA structure remains difficult. Here, we show that coupling systematic mutagenesis with high-throughput chemical mapping enables accurate base-pair inference of domains from ribosomal RNA, ribozymes and riboswitches. For a six-RNA benchmark that has c...
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The three-dimensional conformations of noncoding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure inference with unusually strong validation. We revisit a 16S rRNA domain for which SHAPE (selective 2'-hydroxyl acyla...
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ژورنال
عنوان ژورنال: Nature Chemistry
سال: 2011
ISSN: 1755-4330,1755-4349
DOI: 10.1038/nchem.1176